《植物生理学报》 2015, 51(12): 2169-2174

不同外植体及植物生长调节剂对几种豆科牧草体细胞胚诱导的影响

靳慧卿¹, 米福贵^1,*, 闫利军^2,*, 于洁¹, 贾振宇¹

¹内蒙古农业大学生态环境学院, 呼和浩特010019; ²四川省草原科学研究院, 成都611731

通信作者：米福贵；E-mail: mfguinm@163.com, yanlijun456@126.com；Tel: 15248016137, 0471-4303902

摘　要：

以6个豆科牧草材料的5种不同外植体(幼根、子叶、下胚轴、茎和叶片)为试材, 研究了不同生长调节剂组合下的愈伤组织诱导及体细胞胚分化情况。结果表明: 基因型、外植体类型和植物生长调节剂种类及其浓度均显著影响供试材料愈伤组织的诱导与体细胞胚的分化。不同基因型以‘中苜2号’紫花苜蓿和百脉根整体表现良好, 平均愈伤组织诱导率分别为86.67%和90.13%, 平均体细胞胚分化率分别为36.63%和50.93%; 不同外植体间愈伤组织诱导率及体细胞胚分化率差异较大, 其中下胚轴是理想的受体材料, 表现为出愈早, 体细胞胚形成和再生能力强。供试材料愈伤组织诱导和体细胞胚分化的适宜生长调节剂组合分别为2.0 mg•L^-1 2,4-D+0.5 mg•L^-1 KT和0.1 mg•L^-1 NAA+0.5 mg•L^-1 6-BA。

关键词：豆科牧草; 外植体; 植物生长调节剂; 愈伤组织诱导; 体细胞胚分化

收稿：2015-10-30   修定：2015-11-25

资助：国家“十二五”科技支撑项目(2011BAD17B05-3)和四川省“十二五”饲草育种攻关项目(2011NZ0098-11-3)。

Effects of Different Explants and Plant Growth Regulator Combinations on Somatic Embryo Induction of Leguminous Forage Plants

JIN Hui-Qing¹, MI Fu-Gui^1,*, YAN Li-Jun^2,*, YU Jie¹, JIA Zhen-Yu¹

¹College of Ecology and Environmental Science, Inner Mongolia Agricultural University, Hohhot 010019, China; ²Sichuan Academy of Grassland Science, Chengdu 611731, China

Corresponding author: MI Fu-Gui; E-mail: mfguinm@163.com, yanlijun456@126.com; Tel: 15248016137, 0471-4303902

Abstract:

The effects of different plant growth regulator combinations on callus induction and somatic embryos differentiation of five different explants (radicle, cotyledon, hypocotyl, stem and leaf) of six kinds of leguminous forage were studied. The results show that diverse genotypes, different explants and exogenous plant growth regulators and concentration had obvious effects on callus and somatic embryos induction, in which the genotypes alfalfa (Medicago sativa cv. ‘Zhongmu No.2’) and Lotus corniculatus were better than the others in genotypes. The average callus induction rates from the variety ‘Zhongmu No.2’ of alfalfa and L. corniculatus were 86.67% and 90.13%, respectively, and the average somatic embryo production rates were 36.63% and 50.93%, respectively. There were great differences in callus induction rate and somatic embryo production rate among different explants, and hypocotyl was idealest receptor of all, for forming callus earlier and the stronger regeneration ability in somatic embryo. MS containing 2.0 mg•L^-1 2,4-D and 0.5 mg•L^-1 KT was the optimal embryonic callus induction medium and the medium of MS containing 0.1 mg•L^-1 NAA and 0.5 mg•L^-1 6-BA was the most efficient for inducing somatic embryos.

Key words: leguminous forage plants; explant; plant growth regulator; callus induction; somatic embryos differentiation